Found 50 Enz. Inhib. hit(s) with Target = 'Tyrosine-protein kinase JAK2' and Ligand = 'BDBM50355501' 
Affinity DataKi: 0.100nMAssay Description:Inhibition of human JAK2 (828-1132) expressed in baculovirus-infected Sf9 cells using EQEDEPEGDYFEWLE as substrate after 1 hr by HTRF assayMore data for this Ligand-Target Pair
3D Structure (crystal)Affinity DataKi: 0.100nMAssay Description:Inhibition of JAK2 kinase domain using N-terminal 5-carboxyfluorescein-tagged Val-Ala-Leu-Val-Asp-Gly-Tyr-Phe-Arg-Leu-Thr-Thr as substrate after 30 m...More data for this Ligand-Target Pair
Affinity DataKi: 0.240nMAssay Description:Inhibition of purified JAK2 incubated for 30 minsMore data for this Ligand-Target Pair
Affinity DataIC50: 0.0560nMAssay Description:Inhibition of human JAK2 using poly[Glu:Tyr] as substrate in presence of [gamma-33P]-ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 0.0560nMAssay Description:Inhibition of human JAK2 using poly[Glu:Tyr] (4:1) as substrate in presence of [gamma-33P]ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 0.120nMAssay Description:Inhibition of JAK2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 0.200nMAssay Description:Inhibition of human recombinant JAK2More data for this Ligand-Target Pair
Affinity DataIC50: 0.400nMAssay Description:Inhibition of human JAK2 by radiometric assayMore data for this Ligand-Target Pair
Affinity DataIC50: 0.600nMAssay Description:Inhibition of human recombinant N-terminal hexahistidine tagged JAK2 JH1 catalytic domain (835 to 1132 residues) expressed in baculovirus infected Sf...More data for this Ligand-Target Pair
Affinity DataIC50: 0.700nMAssay Description:Inhibition of JAK2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: <1nMAssay Description:Inhibition of human JAK2 using poly[Glu:Tyr] (4:1) as substrate in presence of [gamma33P]-ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 1.30nMAssay Description:1. Add 20 μl of the diluted compound to each well of the 384-well opti plate2. Tap the plate gently3. Add 10 μl of the enzyme to each well ...More data for this Ligand-Target Pair
Affinity DataIC50: 1.30nMAssay Description:1. Add 20 μl of the diluted compound to each well of the 384-well opti plate2. Tap the plate gently3. Add 10 μl of the enzyme to each well ...More data for this Ligand-Target Pair
Affinity DataIC50: 2nMAssay Description:The assays were performed in 384-well, low volume microtiter assay plates in a final reaction volume of 9 ul. Dose-response curves were generated by ...More data for this Ligand-Target Pair
Affinity DataIC50: 2.70nMAssay Description:Inhibition of JAK2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 2.80nMAssay Description:Inhibition of JAK 2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 2.80nMAssay Description:Inhibition of N-terminal epitope-tagged JAK2 (828 to 1132 residues) (unknown origin) expressed in Sf21 cells using EQEDEPEGDYFEWLE as substrate in pr...More data for this Ligand-Target Pair
Affinity DataIC50: 2.80nMAssay Description:Inhibition of human JAK2 kinase domain expressed in Sf21 cells using EQEDEPEGDYFEWLE as substrate after 1 hr by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 2.80nMAssay Description:Inhibition of JAK2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 2.80nMAssay Description:Inhibition of recombinant human N-terminal epitope-tagged JAK2 (828 to 1132 residues) expressed in baculovirus infected Sf21 insect cells using EQEDE...More data for this Ligand-Target Pair
Affinity DataIC50: 2.80nMAssay Description:Inhibition of human JAK2 (828 to 1132 residues) expressed in baculovirus infected Sf9 insect cells using EQEDEPEGDYFEWLE as substrate after 1 hr by f...More data for this Ligand-Target Pair
Affinity DataIC50: 2.90nMAssay Description:Inhibition of JAK2 (unknown origin) preincubated for 60 mins followed by reagent A addition and measured after 60 mins in presence of ATP by using mi...More data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of human recombinant JAK2 by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of human recombinant JAK2 expressed in Sf21 cells assessed as reduction in Ulight-CAGAGAIETDKEYYTVKD phosphorylation pre-incubated before ...More data for this Ligand-Target Pair
Affinity DataIC50: 3.10nMAssay Description:1. Add 20 μl of the diluted compound to each well of the 384-well opti plate2. Tap the plate gently3. Add 10 μl of the enzyme to each well ...More data for this Ligand-Target Pair
Affinity DataIC50: 3.80nMAssay Description:1. Add 20 μl of the diluted compound to each well of the 384-well opti plate2. Tap the plate gently3. Add 10 μl of the enzyme to each well ...More data for this Ligand-Target Pair
Affinity DataIC50: 4.10nMAssay Description:Inhibition of recombinant human C-terminal 6His-tagged JAK2 (808 to end amino acids) expressed in Sf21 cells measured after 1 hr in presence of ATP b...More data for this Ligand-Target Pair
Affinity DataIC50: 6nMAssay Description:Inhibition of recombinant epitope tagged JAK2 (828 to 1132 residues) (unknown origin) using Chk2 as substrate by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 6.85nMAssay Description:Inhibition of JAK2 in human TF1 cells assessed as reduction in STAT5 phosphorylation incubated for 30 mins in presence of human recombinant EPOMore data for this Ligand-Target Pair
Affinity DataIC50: 8nMAssay Description:Inhibition of JAK2 in human CD34+ cells assessed as inhibition of EPO-mediated cell proliferationMore data for this Ligand-Target Pair
Affinity DataIC50: 8.80nMAssay Description:Inhibition of JAK2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 9nMAssay Description:Inhibition of JAK2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 9nMAssay Description:Inhibition of recombinant human JAK2 using FITC-KGGEEEEYFELVKK as substrate in presence of 1 mM ATP by mobility shift assayMore data for this Ligand-Target Pair
Affinity DataIC50: 9nMAssay Description:Inhibition of JAK2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of recombinant human JAK2 JH1 domain expressed in baculovirus infected insect cells using poly-Glu-Tyr (51 to 68 residues) as substrate in...More data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Inhibition of recombinant human N-terminal GST-tagged JAK2 (831 to 1132 residues) expressed in insect cells using 5FAM-labeled GEEPLYWSFPAKKK-NH2 pep...More data for this Ligand-Target Pair
Affinity DataIC50: 26nMAssay Description:Inhibition of JAK2 in human monocytes expressing CD14 assessed as inhibition of GM-CSF-stimulated STAT5a phosphorylationMore data for this Ligand-Target Pair
Affinity DataIC50: 60nMAssay Description:Inhibition of JAK2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 274nMAssay Description:Inhibition of JAK2 JH2 V617F mutant in human HEL cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 677nMAssay Description:Inhibition of JAK2 homodimer in human CD34+ cells spiked into human whole blood assessed as inhibition of EPO-induced STAT-5 phosphorylation preincub...More data for this Ligand-Target Pair
Affinity DataIC50: 677nMAssay Description:Inhibition of [3H]GR-65630 binding to 5-hydroxytryptamine 3 receptorMore data for this Ligand-Target Pair
Affinity DataIC50: 3.00E+3nMAssay Description:Antagonist potency against histamine H3 receptor in an electrically stimulated guinea pig ileumMore data for this Ligand-Target Pair
Affinity DataIC50: 4.98E+3nMAssay Description:Inhibition of JAK2 in human whole blood assessed as inhibition of GM-CSF-induced pSTAT5More data for this Ligand-Target Pair
Affinity DataKd: 0.0360nMAssay Description:Binding constant for JAK2(JH1domain-catalytic) kinase domainMore data for this Ligand-Target Pair
Affinity DataEC50: 3.80E+3nMAssay Description:Stabilization of human JAK2 KD expressed in Expi293F (HEK293F) cells assessed as increase in JAK2 protein levels incubated for 3 mins by isothermal d...More data for this Ligand-Target Pair
Affinity DataEC50: 12nMAssay Description:Inhibition of JAK2 (unknown origin) assessed as inhibition of STAT5 phosphorylation by cell-based assayMore data for this Ligand-Target Pair
Affinity DataKd: 28nMAssay Description:Binding affinity to human recombinant N-terminal hexahistidine tagged JAK2 JH1 catalytic domain (835 to 1132 residues) expressed in baculovirus infec...More data for this Ligand-Target Pair
TargetTyrosine-protein kinase JAK2(Mus musculus)
Gwangju Institute Of Science And Technology (Gist)
Curated by ChEMBL
Gwangju Institute Of Science And Technology (Gist)
Curated by ChEMBL
Affinity DataEC50: 186nMAssay Description:Inhibition of JAK2 V617F mutant expressed in mouse BaF3 cells cells assessed as reduction in cell viabilityMore data for this Ligand-Target Pair
Affinity DataEC50: 12nMAssay Description:Inhibition of JAK2 in human TF1 cells assessed as inhibition of EPO-induced STAT5 phosphorylation incubated for 20 mins prior to EPO-induction measur...More data for this Ligand-Target Pair
