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Target
E3 ubiquitin-protein ligase Mdm2
Ligand
BDBM31204
Substrate
PMDM6-F Peptide
Meas. Tech.
Fluorescence Polarization-Based (FP-Based) Binding Assay
pH
7.5±n/a
Temperature
296.15±n/a K
Ki
39±5 nM
Citation
 Ding, K; Lu, Y; Nikolovska-Coleska, Z; Wang, G; Qiu, S; Shangary, S; Gao, W; Qin, D; Stuckey, J; Krajewski, K; Roller, PP; Wang, S Structure-based design of spiro-oxindoles as potent, specific small-molecule inhibitors of the MDM2-p53 interaction. J Med Chem 49:3432-5 (2006) [PubMed]  Article 
Target
Name:
E3 ubiquitin-protein ligase Mdm2
Synonyms:
Double minute 2 protein | Double minute 2 protein (HDM2) | E3 ubiquitin-protein ligase Mdm2 (p53-binding protein Mdm2) | Hdm2 | Human Double Minute 2 (HDM2) | MDM2 | MDM2-MDMX | MDM2_HUMAN | p53-Binding Protein MDM2 | p53-binding protein
Type:
Oncoprotein
Mol. Mass.:
55196.54
Organism:
Homo sapiens (Human)
Description:
Q00987
Residue:
491
Sequence:
MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP
  
Inhibitor
Name:
BDBM31204
Synonyms:
spiro-oxindole, 10
Type:
Small organic molecule
Emp. Form.:
C30H37Cl2FN4O2
Mol. Mass.:
575.545
SMILES:
CC(C)(C)C[C@H]1N[C@H]([C@H](c2cccc(Cl)c2F)[C@@]11C(=O)Nc2cc(Cl)ccc12)C(=O)NCCN1CCCCC1 |r|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
PMDM6-F Peptide
Synonyms:
n/a
Type:
Fluorescence probe
Mol. Mass.:
4802.47
Organism:
n/a
Description:
n/a
Residue:
45
Sequence:
FAMETAALAETAALAPHEMETAIPTYRCLLTRPGLACCLEASNNH