Cell Reactant:
PLD beta C2
Syringe Reactant:
BDBM14
Meas. Tech.:
ITC and CD
Entry Date.:
12/04/00
ΔG°:
-26.88±n/a (kJ/mole)
pH:
7.5±n/a
Temperature:
303.15±n/a (K)
ΔH° :
-2.94±n/a (kJ/mole)
ΔHobs :
-2.94±n/a (kJ/mole)
ΔS° :
0.0785±n/a (kJ/mole-K)
Comments:
PLD beta has two classes of binding sites
Citation
 Zheng, LKrishnamoorthi, RZolkiewski, MWang, X Distinct Ca2+ binding properties of novel C2 domains of plant phospholipase dalpha and beta. J Biol Chem 275:19700-6 (2000) [PubMed]  Article
Cell React
Source:
poly-His-fused C2 proteins were expressed in E. coli BL21(DE3)
Prep. Method:
C2 proteins without the tag were refolded by passing through a Sephacryl S-100 gel filtration column.Proteins were dissolved in Chelex-100 treated buffer
Name:
PLD beta C2
Synonyms:
PLD beta C2 domain
Type:
Protein
Mol. Mass.:
121118.74
Organism:
Arabidopsis thaliana
Description:
P93733
Residue:
1083
Sequence:
MDNHGPRYPYPYGQYPYPYPYPAPYRPPSSEPYPPPPTNQYSAPYYPYPPPPYATPPPYASPPPPHQHTSGSHSGPLDYSHNPQPSSLAAAPPEYHRHSFDYQPSPYPYQPQGNFGAYGPPPPHYSYQEPAQYPPPETKPQEPLPPPQQTQGFQEYRRQDCLSTGGTGHDNVSNSGSSYPPVDELLGGLHISTNQPGPSVPQLSSLPSNSWQSRPGDLYGYPNSSFPSNSHLPQLGRVDSSSSYYASTESPHSADMQMTLFGKGSLKVLLLHGNLDIWIYHAKNLPNMDMFHKTLGDMFGRLPGKIEGQLTSKITSDPYVSVSVAGAVIGRTYVMSNSENPVWMQHFYVPVAHHAAEVHFVVKDSDVVGSQLIGLVTIPVEQIYSGAKIEGTYPILNSNGKPCKPGANLSLSIQYTPMDKLSVYHHGVGAGPDYQGVPGTYFPLRKGGTVRLYQDAHVPEGMLPGIRLDNGMSYEHGKCWHDMFDAIRQARRLIYITGWSVWHKVKLIRDKLGPASECTLGELLRSKSQEGVRVLLLIWDDPTSRSILGYKTDGVMATHDEETRRFFKHSSVQVLLCPRNAGKRHSWVKQREVGTIYTHHQKNVIVDADAGGNRRKIIAFVGGLDLCDGRYDTPQHPLFRTLQTIHKDDFHNPTFTGNLSGCPREPWHDLHSKIDGPAAYDVLTNFEERWLKAAKPSGIKKFKTSYDDALLRIDRIPDILGVSDTPTVSENDPEAWHVQIFRSIDSNSVKGFPKDPKDATCKNLVCGKNVLIDMSIHTAYVKAIRAAQHFIYIENQYFIGSSYNWNAHKDIGANNLIPMEIALKIAEKIRANERFAAYIVIPMWPEGVPTGAATQRILYWQHKTIQMMYETIYKALVETGLEGAFSPQDYLNFFCLGNREMVDGIDNSGTGSPSNANTPQALSRKSRRFMVYVHSKGMVVDDEYVVIGSANINQRSMEGTRDTEIAMGAYQPQHTWARKHSGPRGQIYGYRMSLWAEHMATLDDCFTQPESIECVRKVRTMGERNWKQFAAEEVSDMRGHLLKYPVEVDRKGKVRPLPGSETFPDVGGNIVGSFIAIQENLTI
  
Syringe React
Source:
Fisher Scientific
Name:
BDBM14
Synonyms:
Ca2+ | calcium
Type:
Metal Ion
Emp. Form.:
Ca
Mol. Mass.:
40.077
SMILES:
[Ca++]
Structure:
Search PDB for entries with ligand similarity: