| Assay Method Information | |
| | Fluorescence Energy Transfer (FRET) Measurement |
| Description: | The IC50s of compounds were determined in the FRET assay, which was conducted as time-resolved measurement (TR-FRET) in a 1536 well plate format. The Cy5-labeled PGC-1 alpha derived peptide was added to the plates containing the His-ERRalpha LBD. The TR-FRET readout was performed on an Envision 2102 reader (PerkinElmer Life Sciences) with an excitation at 350 nm, first emission (donor) at 615 nm, and second emission (acceptor) at 665 nm. The readout was calculated according to X = donor/acceptor x 1000. Data were fitted with XLfit4 after plate normalization of the derived data. |
| Affinity data for this assay | |
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