| Assay Method Information | |
| | Filtration Binding Assay |
| Description: | Competition experiments were performed in the presence radioligand with membrane protein (obtained from cells expressing the receptor) and test compounds. The mixture was incubated at room temperature for 2-h and the reaction stopped by rapid filtration onto a 96-well GF/B filter using a Packard Filtermate harvester. Radioactivity retained on the filters was measured by liquid scintillation in a Packard TopCount. IC50 values were calculated using a one-site competition fitting model. The inhibition constant (Ki) was calculated from IC50 value by the Cheng and Prusoff equation. |
| Affinity data for this assay | |
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