| Assay Method Information | |
| | In vitro MKP-3 Phosphatase Dose Response Confirmation and Secondary Selectivity/Selectivity Assay |
| Description: | The MKP-3 Phosphatase Secondary Selectivity Assay has been developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH-76390 In vitro HTS assay for MKP-3, Assay Provider Dr. John S. Lazo, Department of Pharmacology at the University of Pittsburgh. The 107 protein tyrosine phosphatases (PTPs) found in the human genome are defined by the active site sequence C(X)5R(S/T), with X being any amino acid, and they are critical regulators of mammalian cell proliferation, differentiation, and apoptosis. PTPs can be divided into at least four subfamilies based on their tertiary structure: Classical phosphotyrosine specific PTPs, dual specificity phosphatases (DSPase), Cdc25 phosphatases and low molecular weight PTPs. One subgroup of the DSPase family are the MAPK phosphatases (MKP), which dephosphorylate MAPKs on threonine and tyrosine residues. Nine members of the DSPases have been c |
| Affinity data for this assay | |
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