| Assay Method Information | |
| | S100A9-RAGE inhibition assay |
| Description: | Incubation of Biot-hS100A9+Compound Samples and rhRAGE-Fc-Acceptor Beads in OptiplateWhen the biot-hS100A9+ compound incubation is finished the solutions are transferred to Optiplate (Optiplate 384 white, Perkin Elmer no. 6007299) and rhRAGE-Fc-Acceptor bead solution is added to each well (use green filtered light). The plate is covered with a plate seal and incubated in the dark in a plate incubator at 25° C. nominally for 40 minutes.Incubation of Biot-hS100A9+Compound Samples and rhRAGE-Fc-Acceptor and Donor Beads in OptiplateAfter incubation Donor bead solution is added to each well (use green filtered light). The plate is covered with a plate seal and incubated in the dark in a plate incubator at 25° C. nominally. After 50 minutes, the plate is incubated (in the dark) on the bench next to the EnVision instrument for 10 minutes, for temperature equilibrium. |
| Affinity data for this assay | |
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