BDBM20608 4-Hydroxytamoxifen::4-Hydroxytamoxifen (9)::4-[(1Z)-1-{4-[2-(dimethylamino)ethoxy]phenyl}-2-phenylbut-1-en-1-yl]phenol::AFIMOXIFENE::CHEMBL489::[3H]4-OHT::cid_449459
SMILES CC\C(=C(/c1ccc(O)cc1)c1ccc(OCCN(C)C)cc1)c1ccccc1
InChI Key InChIKey=TXUZVZSFRXZGTL-QPLCGJKRSA-N
Activity Spreadsheet -- Enzyme Inhibition Constant Data from BindingDB
Found 48 hits for monomerid = 20608
Affinity DataKi: 0.0140nMAssay Description:Antagonistic activity against estrogen receptor beta in presence of 0.1 nM estradiolMore data for this Ligand-Target Pair
Affinity DataKi: 0.0400nMAssay Description:Antagonistic activity against estrogen receptor alpha in presence of 0.1 nM estradiolMore data for this Ligand-Target Pair
Affinity DataKi: 0.160nMAssay Description:In vitro displacement of 0.5 nM [3H]17-beta-estradiol from human Estrogen receptor betaMore data for this Ligand-Target Pair
Affinity DataKi: 0.25nMAssay Description:In vitro displacement of 0.5 nM [3H]17-beta-estradiol from human Estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.390nMAssay Description:Displacement of [3H]estradiol from ERalpha after 4 hrs by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.5nMAssay Description:Binding affinity for estrogen receptor betaMore data for this Ligand-Target Pair
Affinity DataKi: 0.5nMAssay Description:Binding affinity for estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataKi: 1.70nMAssay Description:Binding affinity towards human estrogen receptor beta in a competitive binding assay using fluorescently labelled estradiolMore data for this Ligand-Target Pair
Affinity DataKi: 5.70nMAssay Description:Binding affinity towards human estrogen receptor alpha in a competitive binding assay using fluorescently labelled estradiolMore data for this Ligand-Target Pair
Affinity DataIC50: 1.80E+4nMpH: 7.5Assay Description:In brief, 6 nM purified PldA was incubated with liposomes containing 90 μM 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol), 10 μM...More data for this Ligand-Target Pair
Affinity DataEC50: 16nMAssay Description:Binding affinity for Estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataIC50: 1.35E+3nMAssay Description:Agonist activity at human estrogen related receptor gamma expressed in HEK293 cells by luciferase reporter gene assayMore data for this Ligand-Target Pair
Affinity DataIC50: 4.40E+3nMAssay Description:Antiestrogenic activity at estrogen receptor alpha (unknown origin) expressed in yeast cells assessed as inhibition of 17beta-estradiol-induced beta-...More data for this Ligand-Target Pair
Affinity DataIC50: 5.60nMAssay Description:Inhibition of ERalpha (unknown origin) by fluorescence polarization-based competition binding assayMore data for this Ligand-Target Pair
Affinity DataIC50: 0.794nMAssay Description:Antagonist activity at progesterone receptor in human MCF cells assessed as estradiol-induced receptor responseMore data for this Ligand-Target Pair
Affinity DataIC50: <1.00E+5nMAssay Description:Agonist activity at progesterone receptor in human MCF7 cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 4.90E+3nMAssay Description:Antagonist activity at ERalpha receptor in human MCF7 cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 0.309nMAssay Description:Binding affinity to ERalpha receptor (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 40nMAssay Description:Displacement of fluorescein labeled estradiol from human recombinant ERalpha expressed in baculovirus infected insect cells by fluorescence polarizat...More data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Displacement of fluorescein labeled estradiol from human recombinant ERbeta expressed in baculovirus infected insect cells by fluorescence polarizati...More data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:Displacement of [3H]-E2 from human ER-alpha incubated for 16 to 20 hrs by liquid scintillation counting analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 100nMAssay Description:Displacement of [3H]-E2 from human ER-beta incubated for 16 to 20 hrs by liquid scintillation counting analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 5.30E+5nMAssay Description:Inhibition of human recombinant placental aromataseMore data for this Ligand-Target Pair
Affinity DataIC50: 9.33E+3nMAssay Description:Inhibitory concentration against recombinant rat androgen receptor expressed in Escherichia coli using [3H]methyltrienolone (R 1881)More data for this Ligand-Target Pair
Affinity DataEC50: 6nMAssay Description:In vitro displacement of 3 nM [3H]4-hydroxytamoxifen from estrogen receptor of rat uterine cystolMore data for this Ligand-Target Pair
Affinity DataIC50: 2.60nMAssay Description:Inhibition of estradiol-stimulated MCF-7 breast adenocarcinoma cell proliferationMore data for this Ligand-Target Pair
TargetSteroid hormone receptor ERR2(Homo sapiens (Human))
The Beckman Research Institute
Curated by ChEMBL
The Beckman Research Institute
Curated by ChEMBL
Affinity DataIC50: 650nMAssay Description:Inverse agonist activity at GAL4-DNA binding domain-fused human ERRbeta ligand binding domain expressed in African green monkey CV1 cells assessed as...More data for this Ligand-Target Pair
Affinity DataIC50: 600nMAssay Description:Inverse agonist activity at GAL4-DNA binding domain-fused mouse ERRgamma ligand binding domain expressed in African green monkey CV1 cells assessed a...More data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Antagonist activity at ERalpha in human MCF7 cells assessed as inhibition of estrogen-induced transcription preincubated overnight followed by estrog...More data for this Ligand-Target Pair
Affinity DataIC50: 33nMAssay Description:Antagonist activity at estrogen receptor in mouse GT1-7 cells harboring beta-galactosidase reporter gene assessed as reduction in 17beta-estradiol in...More data for this Ligand-Target Pair
Affinity DataIC50: 2.10nMAssay Description:Binding affinity to GST-tagged human ER alpha ligand-binding domain by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 0.790nMAssay Description:Antagonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as reduction in E2-induced ER-alpha-mediated transcrip...More data for this Ligand-Target Pair
Affinity DataIC50: 1.40nMAssay Description:Antagonist activity at estrogen receptor in human T47D cells incubated for 18 hrs by ultra high sensitivity luminescence reporter gene assayMore data for this Ligand-Target Pair
Affinity DataIC50: 2.58E+4nMAssay Description:Inhibition of ERalpha (unknown origin) in MCF7 cells measured after 24 to 48 hrs by FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.70nMAssay Description:Displacement of fluorescent-labeled E2 from LBD of ERbeta (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 2.30nMAssay Description:Antagonist activity at ERalpha (unknown origin) expressed in human U2OS cells assessed as inhibition of E2-induced transactivation by dual luciferase...More data for this Ligand-Target Pair
Affinity DataIC50: 1nMAssay Description:Antagonist activity at ERbeta (unknown origin) expressed in human U2OS cells assessed as inhibition of E2-induced transactivation by dual luciferase ...More data for this Ligand-Target Pair
Affinity DataIC50: 7.5nMAssay Description:Displacement of fluorescent labeled-ligand from ERalpha (unknown origin) by competitive binding assayMore data for this Ligand-Target Pair
Affinity DataIC50: 2.00E+4nMAssay Description:Cells were seeded into 12-well tissue culture plates to reach 90% confluence at the time of assay. All cell types, aside from the HEK293-gfpPLD2 cell...More data for this Ligand-Target Pair
Affinity DataIC50: 6.50E+3nMAssay Description:Cells were seeded into 12-well tissue culture plates to reach 90% confluence at the time of assay. All cell types, aside from the HEK293-gfpPLD2 cell...More data for this Ligand-Target Pair
TargetUbiquitin-conjugating enzyme E2 N(Homo sapiens (Human))
Burnham Center For Chemical Genomics
Curated by PubChem BioAssay
Burnham Center For Chemical Genomics
Curated by PubChem BioAssay
Affinity DataIC50: >2.00E+4nMAssay Description:Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego CA...More data for this Ligand-Target Pair
Affinity DataIC50: 10.3nMpH: 7.4 T: 2°CAssay Description:The receptor-binding assay was conducted using [3H]4-hydroxytamoxifen (4-OHT) as the radioligand. To estimate the binding affinity, the IC50 values (...More data for this Ligand-Target Pair
Affinity DataEC50: 15nMpH: 7.0 T: 2°CAssay Description:Recruitment of the RIP140 NR-box peptide to the ERRgamma ligand binding domain was assayed by FRET detection. Proteins were set up in a one to one ra...More data for this Ligand-Target Pair
Affinity DataIC50: 13nMpH: 7.4 T: 2°CAssay Description:Binding affinity was measured by a scintillation proximity binding assay using [3H]4-OHT (for ERRgamma) or [3H]estradiol (for ERalpha) as radioligand...More data for this Ligand-Target Pair
Affinity DataIC50: 2nMpH: 7.4 T: 2°CAssay Description:Binding affinity was measured by a scintillation proximity binding assay using [3H]4-OHT (for ERRgamma) or [3H]estradiol (for ERalpha) as radioligand...More data for this Ligand-Target Pair
Affinity DataIC50: 2.20nMAssay Description:Displacement of fluorescent-labeled E2 from LBD of ERalpha (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 17nM EC50: 10.3nMpH: 7.4 T: 2°CAssay Description:Radioligand binding assay was performed by using 96-well microtiterplates containing ER, 17beta-estradiol, and the test compound to be tested and SPA...More data for this Ligand-Target Pair
Affinity DataIC50: 21nM EC50: 32nMT: 2°CAssay Description:Radioligand binding assay was performed by using 96-well microtiterplates containing ER, 17beta-estradiol, and the test compound to be tested and SPA...More data for this Ligand-Target Pair