Solution Information | help | |
Enzyme: | RAC-beta serine/threonine-protein kinase | |
inhibitor: | BDBM24875 | |
substrate: | biotinylated Akt peptide substrate | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.5 | |
Temp. Prep.: | 22 | |
Comments: | n/a | |
Solute 1: | beta-glycerol phosphate | |
Concentration: | 2 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | KCl | |
Concentration: | 50 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | EDTA | |
Concentration: | 0.1 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | MgCl2 | |
Concentration: | 10 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 5: | ATP | |
Concentration: | 150 uM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 1: | water | |
Concentration: | 1.0 | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 2: | DMSO | |
Concentration: | 2.5% | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | HEPES | |
Concentration: | 50 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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