| Solution Information | help | |
| Enzyme: | Transthyretin | |
| inhibitor: | BDBM58653 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose responses curves for compounds identified as active in a set of previous experiments entitled, "Luminescence-based cell-based primary high throughput screening assay to identify activators of Transthyretin (TTR) transcription" (PubChem AID 1117267), "Luminescence-based cell-based high throughput confirmation assay to identify activators of Transthyretin (TTR) transcription" (PubChem AID 1117280) and "Counterscreen for activators of Transthyretin (TTR) transcription: Luminescence-based cell-based high throughput assay to identify inhibitors of Transthyretin (TTR) transcription in HuH7 hepatoma cells" (PubChem AID 1117279) are active by inhibiting hepatic or peripheral TTR levels in HuH7 cells. This assay is performed in a similar manner as the TTR primary screen, except that it employs HuH7 human hepatoma cells. In this assay, the HuH7 cells stably expressing the Gaussia luciferase (eGLuc2) gene under the control of 2kb of | |
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