Reaction Details
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Cell Reactant:
Streptavadin(N23E)
Syringe Reactant:
BDBM12
Meas. Tech.:
ITC
Entry Date.:
12/19/00
pH:
7±n/a
Temperature:
298.15±n/a (K)
ΔH° :
-24.02±n/a (kJ/mole)
ΔHobs :
-24.02±n/a (kJ/mole)
Citation
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Cell React
Source:
Protein was expressed in a T7 expression system (pET-21a, Novagen Inc.,Madison,WI) as insoluble inclusion bodies.
Prep. Method:
Refolded protein was purified by affinity chromatography with iminobiotin-agarose.
Name:
Streptavadin(N23E)
Synonyms:
streptavidin (N23E)
Type:
Protein
Mol. Mass.:
16504.73
Organism:
Streptomyces avidinii
Description:
Biotin-Binding Protein
Residue:
159
Sequence:
DPSKDSKAQVSAAEAGITGTWYEQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ