Reaction Details Report a problem with these data
Cell Reactant:
Trypsin
Syringe Reactant:
BDBM776
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
06/15/04
ΔG°:
-5.43±0.05 (kcal/mole)
pH:
8±n/a
Log10Kb:
3
Temperature:
298.15±n/a (K)
ΔH° :
-1.67±0.1 (kJ/mole)
ΔHobs :
-1.67±0.1 (kJ/mole)
Corrected for ΔHioniz:
not known
ΔS° :
0.01±0 (kJ/mole-K)
Comments:
Since the inhibitor contains some NH4Cl, control experiment was performed with 10 wt % NH4Cl added to a benzamidinium chloride solution at the usual concentration. Within error, the thermodynamic parameters were identical. Therefore, the concentration of inhibitor, based on weight, can be safely corrected for the amount of NH4Cl present.
Citation
Talhout, R; Villa, A; Mark, AE; Engberts, JB Understanding binding affinity: a combined isothermal titration calorimetry/molecular dynamics study of the binding of a series of hydrophobically modified benzamidinium chloride inhibitors to trypsin. J Am Chem Soc 125:10570-9 (2003) [PubMed] Article
Cell React
Source:
no
Name:
Serine protease 1
Synonyms:
Beta-Trypsin | Cationic trypsin | PRSS1 | TRP1 | TRY1 | TRY1_BOVIN | TRYP1 | Trypsin | Trypsin I
Type:
Enzyme
Mol. Mass.:
25790.52
Organism:
Bovine
Description:
P00760
Residue:
246
Sequence:
MKTFIFLALLGAAVAFPVDDDDKIVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYKSGIQVRLGEDNINVVEGNEQFISASKSIVHPSYNSNTLNNDIMLIKLKSAASLNSRVASISLPTSCASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNMFCAGYLEGGKDSCQGDSGGPVVCSGKLQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIASN