Reaction Details 
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Report a problem with these dataCell Reactant:
HIV-1 Protease Mutant, A-1
Syringe Reactant:
BDBM518
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2004-06-23
ΔG°:
-12.3±n/a (kcal/mole)
pH:
5.0000±n/a
Log10Kb:
8.3
Temperature:
298.1500±n/a (K)
ΔH° :
4.8±n/a (kJ/mole)
ΔHobs :
4.8±n/a (kJ/mole)
Corrected for ΔHioniz:
not known
ΔS° :
0.06±n/a (kJ/mole-K)
Comments:
In displacement titration experiments, inhibitors were injected into the calorimetric cell containing the protein prebound to a weak inhibitor, acetylpepstatin
Citation
Muzammil, S; Muzammil, S; Ross, P; Ross, P; Freire, E; Freire, E A major role for a set of non-active site mutations in the development of HIV-1 protease drug resistance. Biochemistry 42:631-8 (2003) [PubMed] ArticleCell React
Source:
Plasmid-encoded HIV-1 protease was expressed in E. coli cells.
Purity:
99%
Prep. Method:
HIV-1 protease was purified and refolded from E. coli inclusion bodies.
Name:
Dimer of Gag-Pol polyprotein [489-587,Q496K,I573V]
Synonyms:
HIV-1 Protease Mutant, A-1
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
Gag-Pol polyprotein [489-587,Q496K,I573V]
Synonyms:
HIV-1 Protease Mutant, A-1 chain A | HIV-1 Protease Mutant(Q7K/I84V) | HIV-1 Protease Mutant, A-1 chain B | POL_HV1H2 | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
10768.18
Organism:
Human immunodeficiency virus type 1
Description:
All of the protease constructs in this study are on the wild type containing the single Q7K mutation to prevent autocatalysis.This protein has enzymatic properties similar to that of the wild type protease.
Residue:
99
Sequence:
PQVTLWKRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNVIGRNLLTQIGCTLNF
Component 2
Name:
Gag-Pol polyprotein [489-587,Q496K,I573V]
Synonyms:
HIV-1 Protease Mutant, A-1 chain A | HIV-1 Protease Mutant(Q7K/I84V) | HIV-1 Protease Mutant, A-1 chain B | POL_HV1H2 | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
10768.18
Organism:
Human immunodeficiency virus type 1
Description:
All of the protease constructs in this study are on the wild type containing the single Q7K mutation to prevent autocatalysis.This protein has enzymatic properties similar to that of the wild type protease.
Residue:
99
Sequence:
PQVTLWKRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNVIGRNLLTQIGCTLNF
Syringe React
Source:
Purified from commercial capsules
Prep. Method:
Further purified by HPLC using a semipreparative C-18 reversed-phase column developed with 0-100% acetonitrile in 0.05% TFA
Name:
BDBM518
Synonyms:
Viracept | CHEMBL584 | (3S,4aS,8aS)-N-tert-butyl-2-[(2R,3R)-2-hydroxy-3-[(3-hydroxy-2-methylphenyl)formamido]-4-(phenylsulfanyl)butyl]-decahydroisoquinoline-3-carboxamide | AG-1343 | Nelfinavir
Type:
Small organic molecule
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
