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Acetate Operon Repressor (C-IclR)
Isothermal Titration Calorimetry
Corrected for ΔHioniz:
Lorca, GL; Ezersky, A; Lunin, VV; Walker, JR; Altamentova, S; Evdokimova, E; Vedadi, M; Bochkarev, A; Savchenko, A Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator. J Biol Chem 282:16476-91 (2007) [PubMed] Article
His-tagged fusion protein was overexpressed and purified in E. coli.
The His6 tag was cleaved from the protein by tobacco etch virus protease. The cleaved protein was then resolved by flowing the mixture through a second Ni2+ column. The purified proteins were dialyzed and concentrated.
Acetate Operon Repressor (IclR)