Cell Reactant:
Major Urinary Protein (MUP-1)
Syringe Reactant:
BDBM22607
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
06/15/08
ΔG°:
-9.28±0.05 (kcal/mole)
pH:
7.4±n/a
Log10Kb:
6.1
Temperature:
300±n/a (K)
ΔHobs :
-15.22±0.1 (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
0.98
ΔS° :
-0.02±0 (kJ/mole-K)
Comments:
Kd value was obtained from J. Mol. Biol. (2006) 362,994-1003.
Citation
 Malham, RJohnstone, SBingham, RJBarratt, EPhillips, SELaughton, CAHomans, SW Strong solute-solute dispersive interactions in a protein-ligand complex. J Am Chem Soc 127:17061-7 (2005) [PubMed]  Article
Cell React
Source:
The MUP-1 gene was cloned and expressed in E. coli cells.
Prep. Method:
MUP-I was purified using Ni-NTA resin. To remove endogenous bound ligands, an ethanol precipitation step was utilized involving addition of 2 volumes of absolute ethanol per volume of protein solution, followed by incubation at 4 C for 2 h. After centrifugation at 5000g, the pellet was lyophilised, resuspended in PBS pH 7.4, and dialyzed overnight against the same buffer.
Name:
Major Urinary Protein (MUP-1)
Synonyms:
MUP1_MOUSE | Major urinary protein 1 precursor | Mup1
Type:
Other Protein Type
Mol. Mass.:
20640.17
Organism:
Mus musculus (mouse)
Description:
n/a
Residue:
180
Sequence:
MKMLLLLCLGLTLVCVHAEEASSTGRNFNVEKINGEWHTIILASDKREKIEDNGNFRLFLEQIHVLENSLVLKFHTVRDEECSELSMVADKTEKAGEYSVTYDGFNTFTIPKTDYDNFLMAHLINEKDGETFQLMGLYGREPDLSSDIKERFAQLCEKHGILRENIIDLSNANRCLQARE
  
Syringe React
Name:
BDBM22607
Synonyms:
1-Nonanol | Nonan-1-ol
Type:
Small organic molecule
Emp. Form.:
C9H20O
Mol. Mass.:
144.2545
SMILES:
CCCCCCCCCO
Structure:
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