Reaction Details  Report a problem with these data
Cell Reactant:
Transcriptional Regulator TtgR-DNA
Syringe Reactant:
BDBM23447
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
07/23/08
ΔG°:
-26.6684±0.0836 (kJ/mole)
pH:
7±n/a
Log10Kb:
3
Temperature:
303.15±n/a (K)
ΔHobs :
-47.234±0.6688 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
-0.0678±0.0022 (kJ/mole-K)
Citation
 Terán, W; Krell, T; Ramos, JL; Gallegos, MT Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression. J Biol Chem 281:7102-9 (2006) [PubMed]  Article
Cell React
Source:
TtgR protein was over-expressed and purified from E. coli. DNA fragment was obtained by annealing two complementary oligonucleotides.
Prep. Method:
Purified TtgR was placed into the ITC cell and titrated by the addition of the 40-bp DNA fragment. The completeness of protein saturation with DNA was verified by polyacrylamide gel electrophoresis.
Name:
Transcriptional Regulator TtgR-DNA
Synonyms:
n/a
Type:
DNA Complex
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
Transcriptional Regulator TtgR
Synonyms:
HTH-type transcriptional regulator ttgR | TTGR_PSEPT | Toluene efflux pump ttgABC operon repressor | ttgR
Type:
Repressor; homodimer
Mol. Mass.:
23852.57
Organism:
Pseudomonas putida
Description:
n/a
Residue:
210
Sequence:
MVRRTKEEAQETRAQIIEAAERAFYKRGVARTTLADIAELAGVTRGAIYWHFNNKAELVQALLDSLHETHDHLARASESEDEVDPLGCMRKLLLQVFNELVLDARTRRINEILHHKCEFTDDMCEIRQQRQSAVLDCHKGITLALANAVRRGQLPGELDAERAAVAMFAYVDGLIRRWLLLPDSVDLLGDVEKWVDTGLDMLRLSPALRK
  
Component 2
Name:
TtgR Operator-Containing DNA Fragment
Synonyms:
n/a
Type:
Duplex DNA
Mol. Mass.:
3377.80
Organism:
n/a
Description:
n/a
Residue:
40
Sequence:
CAGCAGTATTTACAAACAACCATGAATGTAAGTATATTCC
 
Syringe React
Prep. Method:
All chemicals were manipulated in glass vessels and effector samples were neither degassed nor filtered, to avoid evaporation or nonspecific binding.
Name:
BDBM23447
Synonyms:
2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide | Chloramphenicol | Chlornitromycin | Chloromycetin
Type:
Small organic molecule
Emp. Form.:
C11H12Cl2N2O5
Mol. Mass.:
323.129
SMILES:
OC[C@@H](NC(=O)C(Cl)Cl)[C@H](O)c1ccc(cc1)[N+]([O-])=O
Structure:
Search PDB for entries with ligand similarity: