Reaction Details Report a problem with these data
Target
Histone deacetylase 4 [653-1084,H976Y]
Ligand
BDBM25146
Substrate
BDBM25143
Meas. Tech.
HDAC Activity Assay
Temperature
298.15±n/a K
IC50
6000±n/a nM
Comments
extracted
Citation
 Jones, P; Altamura, S; De Francesco, R; Paz, OG; Kinzel, O; Mesiti, G; Monteagudo, E; Pescatore, G; Rowley, M; Verdirame, M; Steinkühler, C A novel series of potent and selective ketone histone deacetylase inhibitors with antitumor activity in vivo. J Med Chem 51:2350-3 (2008) [PubMed]  Article 
Target
Name:
Histone deacetylase 4 [653-1084,H976Y]
Synonyms:
HDAC4 | HDAC4_HUMAN | Histone Deacetylase 4 (HDAC4) Mutant (H976Y) | Histone deacetylase 4 gain of function (GOF) mutant | KIAA0288
Type:
Catalytic domain
Mol. Mass.:
46476.72
Organism:
Homo sapiens (Human)
Description:
His-tagged HDAC4 GOF(gain of function, H976Y) catalytic domains (T653-L1084) was expressed in E.coli and purified by Nickel-Chelation affinity chromatography and anion exchange (MonoQ) chromatography.
Residue:
432
Sequence:
TTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSKKLLGSLASVFVRLPCGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQAFYSDPSVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSARCFGYLTKQLMGLAGGRIVLALEGGYDLTAICDASEACVSALLGNELDPLPEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGRSLIEAQTCENEEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL
  
Inhibitor
Name:
BDBM25146
Synonyms:
2-(5-methoxy-2-methyl-1H-indol-3-yl)-N-[(1S)-7-oxo-1-(5-phenyl-1H-imidazol-2-yl)octyl]acetamide | 4-phenylimidazole, 17 | CHEMBL407959
Type:
Small organic molecule
Emp. Form.:
C29H34N4O3
Mol. Mass.:
486.6053
SMILES:
COc1ccc2[nH]c(C)c(CC(=O)N[C@@H](CCCCCC(C)=O)c3nc(c[nH]3)-c3ccccc3)c2c1 |r|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM25143
Synonyms:
Fluor-de-Lys Fluorogenic Deacetylase Substrate | KI-104 (BioMol Research Laboratories)
Type:
Fluorogenic Substrate
Emp. Form.:
n/a
Mol. Mass.:
n/a
SMILES:
n/a
Structure: