Reaction Details Report a problem with these data
Target
Tyrosine-protein phosphatase non-receptor type 1 [L119V]
Ligand
BDBM13816
Substrate
BDBM13594
Meas. Tech.
PTP1B and TCPTP Inhibition Assay
pH
6.3±n/a
Temperature
295.15±n/a K
IC50
1903±224 nM
Km
19000±2000 nM
Comments
kcat/Km= 71000 M-1s-1
Citation
 Asante-Appiah, E; Patel, S; Desponts, C; Taylor, JM; Lau, C; Dufresne, C; Therien, M; Friesen, R; Becker, JW; Leblanc, Y; Kennedy, BP; Scapin, G Conformation-assisted inhibition of protein-tyrosine phosphatase-1B elicits inhibitor selectivity over T-cell protein-tyrosine phosphatase. J Biol Chem 281:8010-5 (2006) [PubMed]  Article 
Target
Name:
Tyrosine-protein phosphatase non-receptor type 1 [L119V]
Synonyms:
PTN1_HUMAN | PTP-1B | PTP1B | PTPN1 | PTPase 1B | Protein-Tyrosine Phosphatase 1B (PTP1B) Mutant (L119V) | Tyrosine-protein phosphatase, non-receptor type 1
Type:
Enzyme
Mol. Mass.:
34656.62
Organism:
Homo sapiens (Human)
Description:
P18031[L119V] MDYKDDDDKLEF removed
Residue:
298
Sequence:
MEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSVKCAQYWPQKEEKEMIFEDTNLKLTLISEDIKSYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRESGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHED
  
Inhibitor
Name:
BDBM13816
Synonyms:
[difluoro({4-[3-(4-fluorophenyl)-2-[4-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl]-3-oxo-2-[(2E)-3-phenylprop-2-en-1-yl]propyl]phenyl})methyl]phosphonic acid | benzotriazole phenyldifluoromethylphosphonic acid compound 3
Type:
Small organic molecule
Emp. Form.:
C34H28F3N2O5P
Mol. Mass.:
632.5655
SMILES:
Cc1noc(n1)-c1ccc(cc1)C(C\C=C\c1ccccc1)(Cc1ccc(cc1)C(F)(F)P(O)(O)=O)C(=O)c1ccc(F)cc1
Structure:
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Substrate
Name:
BDBM13594
Synonyms:
Fluorescein diphosphate (FDP) | ammonium 6'-(phosphonatooxy)-3H-spiro[2-benzofuran-1,9'-xanthene]-3-one phosphate | fluorescein diphosphate, tetraammonium salt
Type:
Small organic molecule
Emp. Form.:
C20H10O11P2
Mol. Mass.:
488.2365
SMILES:
[O-]P([O-])(=O)Oc1ccc2c(Oc3cc(OP([O-])([O-])=O)ccc3C22OC(=O)c3ccccc23)c1
Structure:
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