Reaction Details Report a problem with these data
Target
cAMP-dependent protein kinase catalytic subunit alpha
Ligand
BDBM14031
Substrate
Kemptide
Meas. Tech.
Kinase Activity Assay
pH
7.6±n/a
Temperature
303.15±n/a K
Ki
2200±n/a nM
Citation
 Jacobs, M; Hayakawa, K; Swenson, L; Bellon, S; Fleming, M; Taslimi, P; Doran, J The structure of dimeric ROCK I reveals the mechanism for ligand selectivity. J Biol Chem 281:260-8 (2006) [PubMed]  Article 
Target
Name:
cAMP-dependent protein kinase catalytic subunit alpha
Synonyms:
KAPCA_MOUSE | PKA C-alpha | Pkaca | Prkaca | cAMP-Dependent Protein Kinase (PKA) | cAMP-dependent protein kinase catalytic subunit alpha | cAMP-dependent protein kinase, alpha-catalytic subunit
Type:
Enzyme Catalytic Unit
Mol. Mass.:
40579.18
Organism:
Mus musculus (mouse)
Description:
n/a
Residue:
351
Sequence:
MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF
  
Inhibitor
Name:
BDBM14031
Synonyms:
5-(1,4-diazepane-1-sulfonyl)-1,2-dihydroisoquinolin-1-one | CHEMBL1233300 | HA-1100 | Hydroxyfasudil
Type:
Small organic molecule
Emp. Form.:
C14H17N3O3S
Mol. Mass.:
307.368
SMILES:
O=c1[nH]ccc2c(cccc12)S(=O)(=O)N1CCCNCC1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Kemptide
Synonyms:
Aurora Peptide Substrate
Type:
Peptide
Mol. Mass.:
773.92
Organism:
n/a
Description:
50 uM ATP as co-substrate.
Residue:
7
Sequence:
LRRASLG