Reaction Details Report a problem with these data
Target
Mitogen-activated protein kinase 14 [G110A]
Ligand
BDBM13336
Substrate
biotinylated GST-ATF2
Meas. Tech.
Enzyme Inhibition Scintillation Proximity Assay
pH
7±n/a
Temperature
303.15±n/a K
IC50
37±n/a nM
Km
85000±12000 nM
Citation
 Fitzgerald, CE; Patel, SB; Becker, JW; Cameron, PM; Zaller, D; Pikounis, VB; O'Keefe, SJ; Scapin, G Structural basis for p38alpha MAP kinase quinazolinone and pyridol-pyrimidine inhibitor specificity. Nat Struct Biol 10:764-9 (2003) [PubMed]  Article 
Target
Name:
Mitogen-activated protein kinase 14 [G110A]
Synonyms:
Crk1 | Csbp1 | Csbp2 | MAP Kinase p38 alpha Mutant (G110A) | MK14_MOUSE | Mapk14 | Mitogen-activated protein kinase 14 | Mitogen-activated protein kinase p38 alpha
Type:
Enzyme
Mol. Mass.:
41295.24
Organism:
Mus musculus (mouse)
Description:
Mutant was site-directed generated from wild-type p38alpha.
Residue:
360
Sequence:
MSQERPTFYRQELNKTIWEVPERYQNLSPVGSGAYGSVCAAFDTKTGHRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMAADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLAQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES
  
Inhibitor
Name:
BDBM13336
Synonyms:
4-[4-(4-fluorophenyl)-2-(4-methanesulfinylphenyl)-1H-imidazol-5-yl]pyridine | 4-[4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-1H-imidazol-5-yl]pyridine | 4-{4-(4-fluorophenyl)-2-[4-(methylsulfinyl)phenyl]-1H-imidazol-5-yl}pyridine | CHEMBL10 | SB-203580 | SB203580 | cid_176155
Type:
Small organic molecule
Emp. Form.:
C21H16FN3OS
Mol. Mass.:
377.435
SMILES:
CS(=O)c1ccc(cc1)-c1nc(c([nH]1)-c1ccc(F)cc1)-c1ccncc1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
biotinylated GST-ATF2
Synonyms:
ATF-2-GST | ATF2 | Activating transcription factor 2 | Activating transcription factor 2-GST fusion | GST-ATF2 | biotinylated ATF2 substrate | biotinylated activating transcription factor 2
Type:
Other Protein Type
Mol. Mass.:
12237.77
Organism:
Homo sapiens (Human)
Description:
n/a
Residue:
109
Sequence:
MSDDKPFLCTAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARNDSVIVADQTPTPTRFLKNCEEVGLFNELASPFENEFKKASEDDIKKMPLDLSPLATPIIRSKIEEPS