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Target
Histone deacetylase 4 [653-1084,H976Y]
Ligand
BDBM25151
Substrate
BDBM25143
Meas. Tech.
HDAC Activity Assay
pH
7.5±n/a
Temperature
310.15±n/a K
IC50
200±n/a nM
Citation
 Jones, P; Altamura, S; De Francesco, R; Gallinari, P; Lahm, A; Neddermann, P; Rowley, M; Serafini, S; Steinkühler, C Probing the elusive catalytic activity of vertebrate class IIa histone deacetylases. Bioorg Med Chem Lett 18:1814-9 (2008) [PubMed]  Article 
Target
Name:
Histone deacetylase 4 [653-1084,H976Y]
Synonyms:
HDAC4 | HDAC4_HUMAN | Histone Deacetylase 4 (HDAC4) Mutant (H976Y) | Histone deacetylase 4 gain of function (GOF) mutant | KIAA0288
Type:
Catalytic domain
Mol. Mass.:
46476.72
Organism:
Homo sapiens (Human)
Description:
His-tagged HDAC4 GOF(gain of function, H976Y) catalytic domains (T653-L1084) was expressed in E.coli and purified by Nickel-Chelation affinity chromatography and anion exchange (MonoQ) chromatography.
Residue:
432
Sequence:
TTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSKKLLGSLASVFVRLPCGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQAFYSDPSVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSARCFGYLTKQLMGLAGGRIVLALEGGYDLTAICDASEACVSALLGNELDPLPEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGRSLIEAQTCENEEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL
  
Inhibitor
Name:
BDBM25151
Synonyms:
9,9,9-trifluoro-8-oxo-N-(3-phenylphenyl)nonanamide | CHEMBL115011 | trifluoromethyl ketone, 11
Type:
Small organic molecule
Emp. Form.:
C21H22F3NO2
Mol. Mass.:
377.4001
SMILES:
FC(F)(F)C(=O)CCCCCCC(=O)Nc1cccc(c1)-c1ccccc1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM25143
Synonyms:
Fluor-de-Lys Fluorogenic Deacetylase Substrate | KI-104 (BioMol Research Laboratories)
Type:
Fluorogenic Substrate
Emp. Form.:
n/a
Mol. Mass.:
n/a
SMILES:
n/a
Structure: