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Target
Baculoviral IAP repeat-containing protein 3 [238-349]
Ligand
BDBM13212
Substrate
Smac-derived peptide
Meas. Tech.
Fluorescence Polarization Assay
pH
7.5±n/a
Temperature
295.15±n/a K
Ki
4.8±1.2 nM
Citation
 Zhang, B; Nikolovska-Coleska, Z; Zhang, Y; Bai, L; Qiu, S; Yang, CY; Sun, H; Wang, S; Wu, Y Design, Synthesis, and Evaluation of Tricyclic, Conformationally Constrained Small-Molecule Mimetics of Second Mitochondria-Derived Activator of Caspases. J Med Chem 51:7352-5 (2008) [PubMed]  Article 
Target
Name:
Baculoviral IAP repeat-containing protein 3 [238-349]
Synonyms:
API2 | Apoptosis inhibitor 2 | BIRC3 | BIRC3_HUMAN | Baculoviral IAP repeat-containing protein 3 | HIAP-1 | Inhibitor of apoptosis protein 1 | MIHC | RNF49 | TNFR2-TRAF-signaling complex protein 1 | cIAP-2 BIR3
Type:
BIR3 domain
Mol. Mass.:
12945.93
Organism:
Homo sapiens (Human)
Description:
Human cIAP2 BIR3 domain (residues 238-349) was used in binding assays.
Residue:
112
Sequence:
QLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQLASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQVQASYPHLLEQLLSTS
  
Inhibitor
Name:
BDBM13212
Synonyms:
(3S,6S,9aS)-N-(diphenylmethyl)-6-[(2S)-2-(methylamino)butanamido]-5-oxo-octahydro-1H-pyrrolo[1,2-a]azepine-3-carboxamide | (3S,6S,9aS)-N-benzhydryl-6-{[(2S)-2-(methylamino)butanoyl]amino}-5-oxooctahydro-1H-pyrrolo[1,2-a]azepine-3-carboxamide | BMC175834 Compound 1b | JMC517169 Compound 13 | JMC517352 Compound 1 | SM-131 | conformationally constrained Smac mimetic 2
Type:
Small organic molecule
Emp. Form.:
C28H36N4O3
Mol. Mass.:
476.6104
SMILES:
CC[C@H](NC)C(=O)N[C@H]1CCC[C@H]2CC[C@H](N2C1=O)C(=O)NC(c1ccccc1)c1ccccc1 |r|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Smac-derived peptide
Synonyms:
SM5F
Type:
fluorescently labelled peptide
Mol. Mass.:
1220.95
Organism:
n/a
Description:
5-carboxyfluorescein was coupled to the lysine side chain of the mutated Smac peptide.
Residue:
10
Sequence:
ARPFKFAMNH