Reaction Details Report a problem with these data
Target
cAMP-dependent protein kinase catalytic subunit alpha
Ligand
BDBM15211
Substrate
Kemptide
Meas. Tech.
Fluorometric TLC Kinase Assay
IC50
100±n/a nM
Citation
 Lavogina, D; Lust, M; Viil, I; König, N; Raidaru, G; Rogozina, J; Enkvist, E; Uri, A; Bossemeyer, D Structural analysis of ARC-type inhibitor (ARC-1034) binding to protein kinase A catalytic subunit and rational design of bisubstrate analogue inhibitors of basophilic protein kinases. J Med Chem 52:308-21 (2009) [PubMed]  Article 
Target
Name:
cAMP-dependent protein kinase catalytic subunit alpha
Synonyms:
KAPCA_MOUSE | PKA C-alpha | Pkaca | Prkaca | cAMP-Dependent Protein Kinase (PKA) | cAMP-dependent protein kinase catalytic subunit alpha | cAMP-dependent protein kinase, alpha-catalytic subunit
Type:
Enzyme Catalytic Unit
Mol. Mass.:
40579.18
Organism:
Mus musculus (mouse)
Description:
n/a
Residue:
351
Sequence:
MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF
  
Inhibitor
Name:
BDBM15211
Synonyms:
CHEMBL104264 | H-89 | H89 | HT-89 (H-89) | N-(2-{[(2E)-3-(4-bromophenyl)prop-2-en-1-yl]amino}ethyl)isoquinoline-5-sulfonamide | N-[2-[[(E)-3-(4-bromophenyl)prop-2-enyl]amino]ethyl]isoquinoline-5-sulfonamide
Type:
Small organic molecule
Emp. Form.:
C20H20BrN3O2S
Mol. Mass.:
446.361
SMILES:
Brc1ccc(\C=C\CNCCNS(=O)(=O)c2cccc3cnccc23)cc1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Kemptide
Synonyms:
Aurora Peptide Substrate
Type:
Peptide
Mol. Mass.:
773.92
Organism:
n/a
Description:
50 uM ATP as co-substrate.
Residue:
7
Sequence:
LRRASLG