Reaction Details Report a problem with these data
Target
Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Ligand
BDBM2583
Substrate
Phosphorylase b
Meas. Tech.
Phosphorylase Kinasse Assay
pH
7.0000±n/a
Temperature
303.1500±n/a K
IC50
3200±400 nM
Citation
 Toullec, D; Pianetti, P; Coste, H; Bellevergue, P; Grand-Perret, T; Ajakane, M; Baudet, V; Boissin, P; Boursier, E; Loriolle, F The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. J Biol Chem 266:15771-81 (1991) [PubMed] 
Target
Name:
Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Synonyms:
Phosphorylase Kinase | KPB1_RABIT | PHKA1
Type:
Enzyme Complex
Mol. Mass.:
138403.25
Organism:
Rabbit
Description:
The enzyme is a hexadecamer of four types of subunits:alpha(4)beta(4)gamma(4)delta(4) isolated from skeletal muscle of rabbits.
Residue:
1237
Sequence:
MRSRSNSGVRLDSYARLVQQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVISFPAFAVEDSKLVEITKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGINVETIAEVYPIRVQPARILSHIYSSLGCNNRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQPTITFPISQTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDNYDELESGDWMDGYNSTSTARCGDEVARYLDHLLAHTAPHPKLAPASQKGGLNRFRAAVQTTCDLMSLVTKAKELHVQNVHMYLPTKLFQASRPSLNLLDSSHPSQEDQVPTVRVEVHLPRDQSGEVDFQALVLQLKETSSLQEQADILYMLYTMKGPDWDTELYEEGSATVRELLTELYGKVGKIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEALTRLIEEACEGDMNISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSISTESQPNGGHSLGADLMSPSFLSPGTSVTPSSGSFPGHHTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ
  
Inhibitor
Name:
BDBM2583
Synonyms:
bis-indolylmaleimide deriv. 8a | 3,4-bis(1H-indol-3-yl)-2,5-dihydro-1H-pyrrole-2,5-dione | 3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione | CHEMBL266487 | Bisindolyl deriv. 3 | Bisindolylmaleimide deriv. 5 | BIM-4
Type:
Small organic molecule
Emp. Form.:
C20H13N3O2
Mol. Mass.:
327.10
SMILES:
O=C1NC(=O)C(=C1c1c[nH]c2ccccc12)c1c[nH]c2ccccc12 |c:5|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Phosphorylase b
Synonyms:
n/a
Type:
Peptide
Mol. Mass.:
1157.29
Organism:
n/a
Description:
250 uM [gamma-32P]ATP as co-substrate.
Residue:
12
Sequence:
ISVGLAGVENVT