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Target
Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B
Ligand
BDBM5594
Substrate
Histone H1
Meas. Tech.
Kinase Inhibition Assay
pH
7.5±n/a
Temperature
303.15±n/a K
IC50
70±5 nM
Citation
 Sayle, KL; Bentley, J; Boyle, FT; Calvert, AH; Cheng, Y; Curtin, NJ; Endicott, JA; Golding, BT; Hardcastle, IR; Jewsbury, P; Mesguiche, V; Newell, DR; Noble, ME; Parsons, RJ; Pratt, DJ; Wang, LZ; Griffin, RJ Structure-based design of 2-arylamino-4-cyclohexylmethyl-5-nitroso-6-aminopyrimidine inhibitors of cyclin-dependent kinases 1 and 2. Bioorg Med Chem Lett 13:3079-82 (2003) [PubMed]  Article 
Target
Name:
Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B
Synonyms:
CDK1/Cyclin B | Cyclin-Dependent Kinase 1 (CDK1) | p34cdc2/Cyclin B
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
p34cdc2/cyclin B was purified from M-phase starfish oocytes by affinity chromatography.
Components:
This complex has 2 components.
Component 1
Name:
Cyclin-dependent kinase 1
Synonyms:
CDK1_ORYLA | Cell division control protein 2 (cdc2) | P34 protein kinase | cdc2 | cdk1
Type:
Enzyme Subunit
Mol. Mass.:
34693.35
Organism:
Marthasterias glacialis (starfish)
Description:
Q9DGD3 {empty hard to find)
Residue:
303
Sequence:
MEDYVKIEKIGEGTYGVVYKGRHKSTGQVVAMKKIRLESEEEGVPSTAVREVSLLQELKHPNVVRLLDVLMQESRLYLIFEFLSMDLKKYLDSIPSGQYMDPMLVKSYLYQILEGIYFCHRRRVLHRDLKPQNLLIDNKGVIKLADFGLARAFGVPVRVYTHEVVTLWYRAPEVLLGSPRYSTPVDVWSTGTIFAELATKKPLFHGDSEIDQLFRIFRTLGTPNNDVWPDVESLPDYKNTFPKWKEGSLSSMVKNLDKNGLDLLAKMLIYNPPKRISAREAMTHPYFDDLDKSTLPAACINGV
  
Component 2
Name:
G2/mitotic-specific cyclin-B
Synonyms:
CCNB_MARGL | Cyclin B | G2/mitotic-specific cyclin-B1
Type:
Enzyme Subunit
Mol. Mass.:
43790.28
Organism:
Marthasterias glacialis (starfish)
Description:
n/a
Residue:
388
Sequence:
MLNGENVDSRIMGKVATRASSKGVKSTLGTRGALENISNVARNNLQAGAKKELVKAKRGMTKSKATSSLQSVMGLNVEPMEKAKPQSPEPMDMSEINSALEAFSQNLLEGVEDIDKNDFDNPQLCSEFVNDIYQYMRKLEREFKVRTDYMTIQEITERMRSILIDWLVQVHLRFHLLQETLFLTIQILDRYLEVQPVSKNKLQLVGVTSMLIAAKYEEMYPPEIGDFVYITDNAYTKAQIRSMECNILRRLDFSLGKPLCIHFLRRNSKAGGVDGQKHTMAKYLMELTLPEYAFVPYDPSEIAAAALCLSSKILEPDMEWGTTLVHYSAYSEDHLMPIVQKMALVLKNAPTAKFQAVRKKYSSAKFMNVSTISALTSSTVMDLADQMC
  
Inhibitor
Name:
BDBM5594
Synonyms:
2-arylamino-pyrimidine deriv. 9d | 4-{[4-amino-6-(cyclohexylmethoxy)-5-nitrosopyrimidin-2-yl]amino}benzamide | CHEMBL101801
Type:
Small organic molecule
Emp. Form.:
C18H22N6O3
Mol. Mass.:
370.4057
SMILES:
NC(=O)c1ccc(Nc2nc(N)c(N=O)c(OCC3CCCCC3)n2)cc1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Histone H1
Synonyms:
Sigma Type IIIS
Type:
Other Protein Type
Mol. Mass.:
358.43
Organism:
Bos taurus (bovine)
Description:
12.5 uM ATP as co-substrate.
Residue:
3
Sequence:
NA