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Target
(S)-6-hydroxynicotine oxidase [Y311F]
Ligand
BDBM217378
Substrate
n/a
Meas. Tech.
Steady-State Kinetic Assay
pH
8±0
Temperature
571.3±0 K
Kd
9.6e+4± 1.8e+4 nM
Citation
 Fitzpatrick, PF; Chadegani, F; Zhang, S; Dougherty, V Mechanism of Flavoprotein l-6-Hydroxynicotine Oxidase: pH and Solvent Isotope Effects and Identification of Key Active Site Residues. Biochemistry 56:869-875 (2017) [PubMed]  Article 
Target
Name:
(S)-6-hydroxynicotine oxidase [Y311F]
Synonyms:
6-hlno | HLNO_PAENI | L-6-hydroxynicotine oxidase (LHNO Y311F)
Type:
Enzyme
Mol. Mass.:
46301.21
Organism:
Arthrobacter nicotinovorans
Description:
A. nicotinovorans LHNO Y311F mutant
Residue:
425
Sequence:
MYDAIVVGGGFSGLKAARDLTNAGKKVLLLEGGERLGGRAYSRESRNVPGLRVEIGGAYLHRKHHPRLAAELDRYGIPTAAASEFTSFRHRLGPTAVDQAFPIPGSEAVAVEAATYTLLRDAHRIDLEKGLENQDLEDLDIPLNEYVDKLDLPPVSRQFLLAWAWNMLGQPADQASALWMLQLVAAHHYSILGVVLSLDEVFSNGSADLVDAMSQEIPEIRLQTVVTGIDQSGDVVNVTVKDGHAFQAHSVIVATPMNTWRRIVFTPALPERRRSVIEEGHGGQGLKILIHVRGAEAGIECVGDGIFPTLFDYCEVSESERLLVAFTDSGSFDPTDIGAVKDAVLYYLPEVEVLGIDYHDWIADPLFEGPWVAPRVGQFSRVHKELGEPAGRIHFVGSDVSLEFPGYIEGALETAECAVNAILHS
  
Inhibitor
Name:
BDBM217378
Synonyms:
(S)-6-hydroxynicotine | 5-[(2S)-1-methylpyrrolidin-2-yl]-1H-pyridin-2-one
Type:
Small organic molecule
Emp. Form.:
C10H14N2O
Mol. Mass.:
178.231
SMILES:
CN1CCC[C@H]1c1ccc(=O)[nH]c1
Structure:
Search PDB for entries with ligand similarity: