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Target
DNA topoisomerase I/type IB small subunit
Ligand
BDBM246595
Substrate
n/a
Meas. Tech.
Stimultaneous Relaxation Assay
pH
7.5±n/a
IC50
4.2e+3± 2.04e+3 nM
Comments
extracted
Citation
 Sharma, G; Chowdhury, S; Sinha, S; Majumder, HK; Kumar, SV Antileishmanial activity evaluation of bis-lawsone analogs and DNA topoisomerase-I inhibition studies. J Enzyme Inhib Med Chem 29:185-9 (2014) [PubMed]  Article 
Target
Name:
DNA topoisomerase I/type IB small subunit
Synonyms:
DNA topoisomerase IB
Type:
Protein
Mol. Mass.:
n/a
Description:
Bi-subunit type IB topoisomerase
Components:
This complex has 2 components.
Component 1
Name:
DNA topoisomerase type IB small subunit
Synonyms:
DNA topoisomerase IB small subunit
Type:
Protein
Mol. Mass.:
28120.90
Organism:
Leishmania donovani
Description:
E9B7X3
Residue:
262
Sequence:
MQPVQSPPVAPPSVPVAAPKKTPIDISALKLKMSPSVRATLAAAGVLGQCPRPIEAADEDALLKLKPMKTMVPSVKKVVAPVATTAPPPKVRRVESSSSSSSDSSSSSSDDDSSSDDSSGSDSSSYSSDDRSSSSAESVVSGEATLFHIAQSQGLVNKEVLTQEEEEVPTLVPPRPPVVRSFPNDIGKALERYRERLNREESIIRIKDDNKAVSLGTSKINYIDPRIICSWAKAQDVPINKIFSATIQKKFPWAMNAENFDF
  
Component 2
Name:
DNA topoisomerase I
Synonyms:
DNA topoisomerase IB large subunit
Type:
Protein
Mol. Mass.:
73610.41
Organism:
Leishmania donovani
Description:
E9BRC0
Residue:
635
Sequence:
MKVENSKMGVKREQSHSNEDEEINEEDLNWWEQENLRIAMKGERRWETLAHNGVLFPPEYEPHGIPIFYDGREFKMTPEEEEVATMFAVMKEHDYYRMEVFRRNFFESWREILDKRQHPIRRLELCDFEPIYQWHLVQREKKLSRTKEEKKAIKEKQDAEAEPYRYCVWDGRREQVANFRVEPPGLFRGRGKHPLMGKLKVRVQPEDITINIGETAEVPVPPAGHKWAAVQHDHTVTWLAMWRDSVAGNMKYVMLAPSSSVKGQSDMVKFEKARKLKDKVDDIRASYMEDFKSNDLHVAQRAVAMYFIDRLALRVGNEKGEDEADTVGCCSLRVEHIQLMPDNIVRFDFLGKDSIRYQNDVAVLPEVYALLQRFTRRKSPGTDIFDQLNPTQLNDHLKSFMDGLSAKVFRTYNASITLDRWFKEKPVDPKWSTADKLAYFNKANTEVAILCNHQKSVSKNFKLQMMQLTTKSEYTRKTIELLEKAEVTAKKKSVEEAAKEFLEEQDRMQREWLESYGTEEQKKEFEEIVAKRAAPRVRSEKKKSTSGAKKAESASGKKRAAKKKKSAKKGGKVLSKKAASKSSKKAPKKLKEEDEDDVPLVSVAAKTKKTAGVKRQRANKVVSDDDDVPLAALRV
  
Inhibitor
Name:
BDBM246595
Synonyms:
Camptothecin (CPT)
Type:
Small organic molecule
Emp. Form.:
C20H18N2O3
Mol. Mass.:
334.3685
SMILES:
CC[C@@]1(O)C(=O)OCC2=CN3Cc4cc5ccccc5nc4C3C=C12 |r,t:8,26|
Structure:
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