Assay Method Information | |
| In Vitro CaMKIIδ Activity Assay |
Description: | The inhibition of CaMKII activity was evaluated using a coupled assay measuring ADP released following ATP hydrolysis and phosphor-transfer to the peptide substrate AC3 (KKALHRQETVDAL; SEQ ID NO: 1) (1). A full length, C-terminal His/Gln tagged CaMKIIδ construct was used. |
Affinity data for this assay | |
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