| Assay Method Information | |
| | IDO Cellular Activity Inhibition Assay |
| Description: | The experimental principle is summarized as follows: IDO expression is low in Hela cells under no induction, but a certain concentration of IFN-γ can induces Hela cells to express IDO which catalyzes the conversion of tryptophan to N-formyl kynurenine, which in turn is hydrolyzed by trichloroacetic acid to give kynurenine. Kynurenine then reacts with the Ehrlich reagent to give a color enabling detection of the IDO activity. The absorbance at 490 nm (OD490) is directly proportional to the IDO activity.The test compound was dissolved in DMSO (Sigma, Cat. No. D5879) and diluted to 5 mM, then serially diluted 3-fold with DMSO to a minimum concentration of 2.29 μM, and each concentration point was further diluted 50-fold with FBS-free DMEM medium (ThermoFisher, Cat. No. 11995073). If a compound's IC50 value was very low, the initial concentration of the compound was lowered. |
| Affinity data for this assay | |
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