| Assay Method Information | |
| | Single Dose Enzymatic Inhibition Assay |
| Description: | To identify potential MBL inhibitors, the relative change in the formation of hydrolyzed nitrocefin between treated and untreated VIM2 was determined as percentage inhibition. 5 nM VIM-2 was pre-incubated for 10 mins with 50 μM of each compound, followed by the addition of 10 μM nitrocefin. The relative change in the λmax=486 nm absorbance after 30 mins was evaluated as percentage inhibition. |
| Affinity data for this assay | |
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