| Assay Method Information | |
| | Alphascreen Assay |
| Description: | Alphascreen Assays to Determine the Apparent Kd Values for Wild-Type and Mutant β-Catenin/BCL9 InteractionsThe experimental detail using AlphaScreen to determine the apparent Kd values for β-catenin/BCL9 and β-catenine/E-cadherin PPIs has been described previously (Zhang et al. (2015) Anal. Biochem. 469: 43-53). The concentrations of mutant β-catenin proteins used in the assays were the same as that of wild-type β-catenin. |
| Affinity data for this assay | |
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