Assay Method Information | |
| ATR Inhibition Assay |
Description: | Compounds were screened for their ability to inhibit ATR kinase using a radioactive-phosphate incorporation assay. Assays were carried out in a mixture of 50 mM Tris/HCl (pH 7.5), 10 mM MgCl2 and 1 mM DTT. Final substrate concentrations were 10 μM [y-33P]ATP (3mCi 33P ATP/mmol ATP, Perkin Elmer) and 800 μM target. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |