| Assay Method Information | |
| | Enzymatic Assay for MASP-2 |
| Description: | The MASP-2 assay protocol is carried out as follows. Test compounds are serially diluted in DMSO and then 100 nL of each dilution is transferred to the assay plate(s). 10 μL of Assay Buffer is added, followed by 15 μL of Enzyme MASP-2 (CCP1-CCP2-SP) in Assay Buffer. 15 μL of Substrate in Assay Buffer is then added and mixed to start the reactions. After 20 min at room temperature, 15 μL of a stop solution (0.1 M acetic acid) is added, mixed and the plates are read on a SpectraMax i3x Microplate Reader and exported as Excel files. Each assay plate included a no inhibitor (DMSO Only) control, a no enzyme control and a reference inhibitor control. % Activity values=100*(average test comp. fluorescence−average no enzyme fluorescence)/(average DMSO only fluorescence−average no enzyme fluorescence). |
| Affinity data for this assay | |
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