| Assay Method Information | |
| | LMPTP Primary Screening Protocol |
| Description: | 1. Prepare Reagents as described in section F. Recipe.2. Using LabCyte Echo, transfer 60 nL from 2 mM test compound source plate into assay plate Col. 5-48 (final concentration of test compounds is 20 μM). 60 nL of DMSO should be transferred to col. 1-4 for positive and negative control wells.3. Spin plates at 1000 rpm for 1 minute in centrifuge.4. Using the Beckman Coulter Bioraptr, add 3 μL/well of control buffer to columns 1 and 2.5. Using the Bioraptr, add 3 μL/well of enzyme solution to col. 3-48.6. Using the Bioraptr, add 3 μL/well of substrate solution to col. 1-48.7. Spin plates at 1000 rpm for 1 minute in centrifuge.8. Incubate plates in the dark at room temperature for 50 minutes.9. Read plates on PerkinElmer Viewlux using a FI protocol. |
| Affinity data for this assay | |
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