| Assay Method Information | |
| | Calcium Channels Interaction |
| Description: | Interaction with N-type calcium channels was assessed with radiolabelled a competitive binding assay, applying the procedure described by Wagner et al. (J. Neurosci. 8: 3354-3359). N-type calcium channels were prepared from rat cerebral cortex, and were incubated with [125I]ω-conotoxin GVIA (0.001 nM). The investigated molecules were added, and the reactants were incubated over 30 minutes at room temperature. Binding shifts were monitored by scintillation counting. |
| Affinity data for this assay | |
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