| Assay Method Information | |
| | In Vitro Enzymatic Activity Inhibition Test (TGFbetaR1) |
| Description: | (Promega), the inhibitory effect of the compounds of the present invention on the enzymatic activity of TGFβR1 was determined, and the steps were as follows: TGFβR1 enzyme were pre-incubated with different concentrations of test compounds (1000 nM, 100 nM, 10 nM) at 30 C. for 30 min, TGFβR1 peptide and adenosine triphosphate (ATP) were added to initiate the reaction. The incubation was performed at 30 C. for 3 h, followed by an addition of ADP-Glo reagent and incubated at room temperature for 90 min, kinase detection reagent was then added. Chemiluminescence signal values were detected after incubation at room temperature for another 30 min. |
| Affinity data for this assay | |
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