| Assay Method Information | |
| | DGAT2 Enzymatic Activity Assay |
| Description: | DGAT2 activity was determined by measuring the amount of enzymatic product 13C18-triolein (13C-1,2,3-Tri(cis-9-octadecenoyl)glycerol) using the membrane prep mentioned above. The assay was carried out in ABgene 384-well assay plates in a final volume of 25 μL at rt. The assay mixture contained the following: assay buffer (100 mM Tris Cl, pH 7.0, 20 mM MgCl2, 5% ethanol), 25 μM of diolein, 5 μM of 13C oleoyl-CoA and 8 ng/μL of DGAT2 membrane. |
| Affinity data for this assay | |
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