Assay Method Information | |
| CYP19 Assay |
Description: | The enzyme activity was assayed by measuring the 3H-labeled H2O formed from [1beta, 2beta-3H] testosterone or [1beta-3H] androstenedione during aromatization. After incubation, the reaction was terminated, and the charcoal-adsorbed steroids were separated by centrifugation. Aliquots of the supernatant were assayed for the presence of 3H-labeled H2O by counting using a liquid scintillation spectrometer. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |