| Assay Method Information | |
| | LpxC Enzyme Assay |
| Description: | Briefly, Pseudomonas aeruginosa LpxC enzyme (0.1 nM) purified from E. coli-overexpressing bacteria was incubated at 25 C. in a final volume of 50 ul containing 0.5 uM UDP-3-O (R-3-hydroxydecanoyl)-N-acetylglucosamine, 1 mg/mL BSA, and 50 mM sodium phosphate buffer, pH 8.0 in the presence and absence of inhibitor compound. At the end of 1 hour, 5 ul of 1 N HCl was added to stop the enzyme reaction, the plates were centrifuged, and then processed with the BioTrove Rapidfire HTMS Mass Spectrometry System. A no-enzyme control was used in calculating the IC50 values from the percent conversion values. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |