| Assay Method Information | |
| | ADP-Glo Assay Promega |
| Description: | The ADP-GloTM Kinase Assay kit from Promega was used to screen the compounds before determination of their IC50 using the radiolabeled kinase assay. This assay kit is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure kinase activity. It quantifies the amount of ADP produced during a kinase reaction. The ADP-GloTM Kinase Assay was performed in a 384-multiwell plate using the manufacturer's protocol. Assays were carried out at room temperature for 10 mins using 25 μM Woodtide and 25 μM ATP in 50 mM HEPES pH 7.5, 50 mM MgCl2, 50 mM DTT. The data was analyzed using GraphPad Prism 8. |
| Affinity data for this assay | |
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