| Assay Method Information | |
| | ATM Biochemical Assay |
| Description: | ATM (Millipore, Cat. No. 14-933) enzyme solution was prepared in 1x kinase base buffer. 10 ul of 2x enzyme solution was transferred to each well of the 384-well assay plate containing 100 nl compounds added by Echo. The plate was incubated at room temperature for 10 minutes. 2x peptide solution was prepared with FAM-labeled peptide and ATP in the 1x kinase base buffer (final concentration: 1.5 nM). 10 ul of 2x peptide solution was added to each well of the 384-well assay plate which was incubated at 37 C. for 210 min before 40 ul stop buffer was added to stop reaction. Data was collected by Caliper. |
| Affinity data for this assay | |
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