| Assay Method Information | |
| | Conditioned place preference (CPP) assay |
| Description: | The CPP assay was performed 6 h later under the ad libitum feeding condition (n=10 mice for both the Vehicle- and ACY775-treated groups). CPP assay under a 20-h fasting condition was also performed. DIG mice were treated with vehicle or ACY775 (10 mg/kg, i.p.) once a day and fasted for 15 h after the third injection. On the fourth day, 1 h after the beginning of the light cycle, the mice were administered vehicle or ACY775 (10 mg/kg body weight, i.p.) and the CPP assay was performed 5 h after this injection under the fasting condition (n=10 mice for both the Vehicle- and ACY775-treated groups). Measurements were made of the total distance that the mice traveled during the CPP test, the average velocity of movement during the assay, the total time the mice spent in the dark chamber during the test; the frequency with which the mice traveled to the dark chamber during the test; the total time the mice spent in the food-paired white chamber; the frequency with which the mice traveled to the food paired white chamber; the total time the mice spent in the food-containing zone within the food-paired side chamber; and the frequency with which the mice traveled to the food-containing zone within the food-paired side chamber during the test. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |