| Assay Method Information | |
| | Inhibitory Activity Assay |
| Description: | Briefly, the compounds were diluted at concentrations of 1000, 333, 111, 37.0, 12.3, 4.12, 1.37, 0.457, 0.152, and 0.0495 nM, respectively, and then a peptide/kinase mixture (final 7.07-28.3 ng p38 alpha, 2 μM serine/threonine 15, 50 mM HEPES pH 7.0, 0.01% NaN3) and ATP solution were added respectively. After reacting at room temperature for 1 hour, a development reagent was added, followed by a reaction again at room temperature for 1 hour. Kinase inhibitory ability was identified at wavelengths of 445 nm and 520 nm. |
| Affinity data for this assay | |
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