| Assay Method Information | |
| | Inhibition Assay |
| Description: | To calculate the IC50 inhibition of Axl and FLT3 by the compounds of the invention, 10 concentrations of each compound 1, 2 and 3 were prepared by 1/3 serial dilutions from the starting concentration. The Axl or FLT3 kinases were diluted at a two-fold concentration in kinase buffer. All the ATP solutions are diluted in a 4-fold working concentration (50 mM HEPES PH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA).Each compound is incubated at 10 concentrations between 100 nM and 0.00495 nM in order to calculate the IC50. |
| Affinity data for this assay | |
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