| Assay Method Information | |
| | Enzyme Activity Assay |
| Description: | Enzyme activity is determined by capturing 14CO2 using an assay described by Kivirikko and Myllyla (1982, Methods Enzymol. 82:245-304). Assay reactions contain 50 mM HEPES (pH 7.4), 100 μM α-ketoglutaric acid sodium salt, 0.30 μCi/mL ketoglutaric acid α-[1-14C]-sodium salt, 40 μM FeSO4, 1 mM ascorbate, 1541.8 units/mL Catalase, with or without 50 M peptide substrate and various concentrations of compound of the invention. Reactions are initiated by addition of HIF-PH enzyme. |
| Affinity data for this assay | |
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