| Assay Method Information | |
| | GR Binding Assay |
| Description: | Binding of test compounds to the glucocorticoid receptor (GR) is determined using a fluorescence polarisation (FP) assay utilising a recombinant ligand binding domain (LBD) of GR. The test compounds are assessed by their ability to displace a fluorescently tagged ligand and detection of the resulting decrease in fluorescence polarisation. Fluorescence polarisation values are converted to % inhibition using the high (1% DMSO only) and low (1 μM) controls and IC50 values are calculated from non-linear regression curves fitted using Dotmatics software. |
| Affinity data for this assay | |
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