| Assay Method Information | |
| | Enzymatic Activity Assay |
| Description: | Human sialidase inhibitors may inhibit the enzymatic activity of all human sialidases, a subset of human sialidases, or one human sialidase, all in wild type form alone or also including one or more active variants. In particular, sialidase inhibitors may inhibit the enzymatic activity of at least human NEU1 alone, human NEU2 alone, human NEU3 alone, or human NEU4 alone. Enzymatic activity may be defined as inhibited if the rate measured by the Michaelis-Menten equation in an in vitro assay using a substrate with a terminal sialic acid is inhibited by at least 50%. More specifically, a human sialidase inhibitor may be a compound that inhibits the rate of at least one human sialidase by at least 50% as measured by the Michaelis-Menten equation in an in vitro assay using the fluorometric substrate 4MU-NANA [2′-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. |
| Affinity data for this assay | |
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