| Assay Method Information | |
| | Evaluation of TTK Activity Inhibitory Assay |
| Description: | A recombinant human TTK protein was mixed with the compound in a reaction solution (8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.33 mg/mL MBP, 10 mM Magnesium acetate, 45 uM) and then reacted at room temperature for 40 minutes. After that, the reaction was terminated by adding phosphoric acid to a concentration of 0.5%, and 10 of the reaction solution was dropped onto a P30 filter. The resultant filter was washed four times with a 0.425% phosphoric acid solution, each wash lasting for 4 minutes, and then washed with methanol once more, and dried. The activity of TTK was measured through scintillation counting. The activity value from the group with no compound was set as 100% of control, and the degree of TTK inhibition by the compound was calculated. |
| Affinity data for this assay | |
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