| Assay Method Information | |
| | Inhibitory Activity Against SARS-COV-2 3CL Protease |
| Description: | 200 nL of 3-fold serially diluted compound (final concentration: 10 μM-0.5 nM) was incubated with 10 μL of 200 nM recombinant 3CL protease (BPS Kit) at room temperature for 30 min. To initiate the reaction, 10 μL of 100 μM fluorescent substrate was added to the reaction plate. Fluorescence signal readings (Ex360/Em460) were taken every 1 minute using Envision (Perkin Elmer) in a continuous reading mode. Fluorescence values were collected over 60 min to calculate the Slope. The inhibition rate of the compound was calculated using the substrate wells as the 100% inhibition control and the enzyme reaction wells as the 0% inhibition control. IC50 was calculated using IDBS XLfit. |
| Affinity data for this assay | |
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