| Assay Method Information | |
| | Inhibitory Activity Assay |
| Description: | A compound serially diluted with DMSO was mixed with a FAK recombinant protein. After the mixture was left to stand at room temperature for 10 min, a biotin-labeled TK substrate (TK) and ATP were added. After 40 min of reaction at room temperature, Sa-XL 665 and a Crytate-labeled TK antibody were added. After 1 h of incubation at room temperature, the fluorescence intensities at 615 nm and 665 nm were measured. The ratio of the fluorescence intensities at 665 nm and 615 nm was calculated. The inhibition percentage and IC50 of the compound were calculated compared to the DMSO control group. |
| Affinity data for this assay | |
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